Biogenesis of prokaryotic membrane proteins

Project leader

Funding source

Swedish Research Council - Vetenskapsrådet (VR)

Project Details

Start date: 01/01/2011
End date: 31/12/2014
Funding: 5170000 SEK


The project has five main aims: (i) to measure the free energy of insertion of model transmembrane segments into the inner membrane of E. coli; (ii) to measure the force exerted on model transmembrane segments during insertion into the inner membrane of E. coli; (iii) to study the mechanism of membrane protein topology determination and evolution in E. coli using dual topology proteins; (iv) to study the cell division process in live E. coli cells using fluorescently labeled proteins, and (v) to improve current topology prediction and targeting signal prediction methods. The project described in this application will give us a better quantitative understanding of membrane protein biogenesis. This is of major importance in a field dominated by qualitative studies. It will also provide important new information on cell division in E. coli. Methods to identify membrane proteins and to predict their topology will continue to be required for all genome annotation efforts. Judging from the heavy use, both in academia and industry, of some of our previously developed prediction methods and the large numbers of citations they receive, we feel that our work in these areas will be of continued relevance to a wide community of researchers.

Last updated on 2017-31-03 at 12:56