Intrecellular protein transport in plants - signal peptides, molecular chaperones, proteolysis


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2007
End date: 31/12/2011
Funding: 2700000 SEK


Description

Organelle biogenesis requires specific intracellular protein transport, sorting, processing and assembly machineries. Newly synthesized organellar proteins contain targeting signals that are recognized by organellar receptors and mediate import into the organelle. Molecular chaperones assist protein import. We combine biochemical and molecular biological approaches with bioinformatics to study intracellular protein transport in plants with emphasis on sorting mechanisms and proteolysis. The project has resulted in several original findings revealing unique features of the plant mitochondrial protein import system. Our studies comprise 1) Sorting of the nuclear encoded precursor proteins between mitochondria and chloroplasts, mapping import determinants with site-directed and deletion/truncation mutants and investigating the role of cytosolic molecular chaperones. 2) Dual targeting of proteins to mitochondria and chloroplasts mapping domain structure and using in vivo high-throughput transient GFP expression. 3) Dual precursor recognition events by processing peptidase integrated into the bc1 complex of the respiratory chain. 4) Mitochondrial molecular chaperones, their partners and interaction with signal peptides. 5) Proteolytic events including Arabidopsis knockout mutants of the newly identified novel mitochondrial and chloroplastic peptidasome degrading targeting peptides. 6) Functional genomics of mitochondrial proteolysis and studies of a rhomboid protease.

Last updated on 2017-24-03 at 12:06