Intracellular protein transport in plants: signal peptides, molecular chaperones and proteolysis


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2010
End date: 31/12/2012
Funding: 2700000 SEK


Description

Organelle biogenesis requires specific intracellular protein transport, sorting, processing and assembly machineries. Newly synthesized organellar proteins contain targeting signals that are recognized by organellar receptors and mediate import into the organelle. Molecular chaperones assist protein import. We combine biochemical and molecular biological approaches with bioinformatics to study intracellular protein transport in plants with emphasis on sorting mechanisms and proteolysis. The project has resulted in several original findings revealing unique features of the plant mitochondrial protein import system. Our studies comprise 1) Sorting of the nuclear encoded precursor proteins between mitochondria and chloroplasts, mapping import determinants with site-directed and deletion/truncation mutants and interaction of signal peptides with organellar receptors. 2) The role of novel cytosolic molecular chaperones and other protein recognition factors involved in protein sorting. 3) Dual targeting of proteins to mitochondria and chloroplasts, mapping domain structure and using in vivo high-throughput transient GFP expression. 4) Studies of interaction of dual targeting peptides with organellar receptors. 5) Proteolytic events including Arabidopsis knockout mutants of the novel mitochondrial and chloroplastic peptidasome degrading targeting peptides. 6) Functional genomics of mitochondrial proteolysis and studies of an intramembrane rhomboid protease.

Last updated on 2017-24-03 at 12:05