Stress tolerance in plants - the role of protein phosphorylation


Project leader


Funding source

Formas - The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning


Project Details

Start date: 01/01/2008
End date: 31/12/2010
Funding: 2360000 SEK


Description

Different forms of stress cause severe limitations in plant productivity. Plants have evolved a number of strategies to minimize the negative effects of stress. The molecular mechanisms of these adaptations are however still poorly understood. The present project aims at a molecular characterization of the environmentally-modulated phosphorylation of proteins related to plant stress resistance. Reversible protein phosphorylation regulates all aspects of cell physiology, development and, particularly, plant photosynthesis. Using the state-of-the-art mass spectrometry techniques and methodologies developed in my laboratory we have presently a world leading position in the mapping and characterization of natural protein phosphorylation reactions occurring in photosynthetic membranes of the model plant Arabidopsis and the model green alga Chlamydomonas. Using quantitative mass spectrometry we will characterize changes in the protein phosphorylation network in plants subjected to high light, drought, low- and high-temperature stress and find the reactions involved in cell signaling and the adaptive restructuring of the photosynthetic protein complexes. We have discovered a new class of proteins that undergo phosphorylation at the surface of the photosynthetic membrane in response to light, drought and to limitation in the environmental carbon dioxide. The functions of these proteins in cell signaling and stress tolerance will be analyzed by proteomics and reverse genetics.


External Partners


Last updated on 2017-24-03 at 12:56