Transcriptional regulation in Drosophila development


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2009
End date: 31/12/2011
Funding: 1680000 SEK


Description

The goal of this research is to understand the molecular mechanisms of gene control that are used in animal development. We have previously identified two novel transcriptional co-repressors required for Drosophila development. One of them, Brakeless, interacts with the causative agent of a neurodegenerative disease, and is required for function of the Tailless repressor. We will further investigate Brakeless’ role in early Drosophila development, in particular, its relation to the Tramtrack69 protein. Chromatin regulators in epigenetic inheritance and embryo development will also be studied. 13 JmjC-domain genes are found in Drosophila, which encode putative histone demethylases. In a cell-culture assay we found that CG15835 reduces histone 3 lysine 36 tri-methylation. A CG15835 mutant has been generated, and its role in epigenetic inheritance and dosage compensation will be investigated. We have demonstrated that histone deacetylation is a mechanism by which the Snail repressor keeps neuroectoderm-specific genes off in the mesoderm, through its interaction with the co-repressor Ebi. The role of Ebi in Huckebein-mediated repression will also be examined. Genetically manipulated embryos together with ChIP assays provide us with a unique opportunity to study regulation of a developmental control gene in vivo. We will explore if control of transcriptional elongation by release from promoter-proximal pausing is a mechanism of regulating developmental programs of gene expression.

Last updated on 2017-31-03 at 12:58