Biogenisis of subunit STT3 and assembly of the OST complex


Project leader


Funding source

Swedish Foundation for International Cooperation in Research and Higher Education (STINT)


Project Details

Start date: 30/08/2010
End date: 10/06/2012
Funding: 190000 SEK


Description

Recently, with grants from STINT, I visited Seoul National University to create a platform for future collaboration with Dr. Hyun Ah Kim and our cooperation between Stockholm University and Seoul National University. Our joint application will benefit both partner universities. I have earlier showed that proteins can be crosslinked to the recently identified STT3 subunit of the oligosaccharyl transferase (OST) complex, referred to as OT in yeast. The mammalian OST and yeast OT complex contains many different subunits and STT3 (Stt3p in yeast) is believed to have the active site. At present, the specific role of each of the individual subunits is unknown and to better explain the mechanism and the function of the OST complex, we want to take advantage of two approaches – first, to examine the assembly of the OST-translocon-ribosome complex (TRC) with and without the crosslinking technique, and second, to study the integration of STT3 into the endoplasmic reticulum (ER) membrane using an insertion model technique. We will give particular emphasis to areas within the multispanning membrane protein STT3 where refinements are required in order to understand and predict protein topogenesis at a molecular level. To start this project and to investigate the assembly of the OST-TRC, we will also examine how the first four weak hydrophobic TM segments of STT3 insert into the membrane using both an integration technique and crosslinking technique with amber codon and charged tRNA, and later extend the study to map its interaction with other subunits in the OST-TRC. We will combine this study with yeast genetics and in vivo crosslinking together with Dr. Hyun Ah Kim at Seoul National University in Korea.


External Partners


Last updated on 2017-24-03 at 12:06