Biogenesis and Overexpression of Membrane Proteins

Project leader

Funding source

Swedish Research Council - Vetenskapsrådet (VR)

Project Details

Start date: 01/01/2010
End date: 31/12/2012
Funding: 2400000 SEK


Integral membrane proteins account for 20-30% of all open reading frames in sequenced pro- and eukaryotic genomes, and they fulfill a wide range of central functions in the cell. However, our knowledge of this important class of proteins is still poor. The biogenesis of membrane proteins has mainly been studied using focused approaches, and functional and structural studies of membrane proteins are strongly hampered by a lack of reliable and robust overexpression systems. Therefore, my laboratory is -i- studying the biogenesis of the cytoplasmic membrane proteome of the bacterium Escherichia coli using a global approach, i.e., a combination of molecular biology and proteomics techniques, and -ii- engineering E. coli strains with improved membrane protein overexpression characteristics. In depth knowledge of membrane protein biogenesis plays a key role in engineering the overexpression strains. Importantly, the model organism E. coli contains the blueprints of membrane protein biogenesis pathways present in many both pro- and eukaryotic systems, making it an ideal model to study the fundamentals of membrane protein biogenesis. Furthermore, it is the most widely used host for overexpressing both pro- and eukaryotic membrane proteins. Our research will provide the first global picture of the different membrane protein biogenesis pathways operational in a model membrane system, and platforms for the efficient production of membrane proteins for functional and structural studies.

Last updated on 2017-31-03 at 12:58