Membrane proteins: Biogenesis, structure, function

Project leader

Funding source

Swedish Research Council - Vetenskapsrådet (VR)

Project Details

Start date: 01/01/2008
End date: 31/12/2010
Funding: 4350000 SEK


The project has five main goals: (i) To deepen our understanding of membrane protein topology determinants, with particular focus on the mechanism of membrane insertion and evolution of ‘dual-topology’ and internally duplicated membrane proteins. (ii) To study the structure and function of the small multi-drug resistance (SMR) proteins. (iii) To develop a new method for determining the location of the N-terminus of bacterial membrane proteins and to apply this method to a large set of E. coli membrane proteins. (iv) To determine the quantitative ‘rules’ for the recognition of transmembrane helices in E. coli. (v) To develop methods for predicting membrane protein topology and structure based on experimentally measured parameters. The new openings provided by our recent work on membrane protein evolution, small multi-drug resistance proteins, and the energetics of transmembrane helix formation will make it possible to understand membrane protein assembly, topology, and structure in much greater detail than before and will help us improve topology- and structure-prediction algorithms. Judging from the heavy use of our previously developed prediction methods (SignalP and TMHMM are part of the SwissProt/UniProt annotation pipeline and are run in-house by many pharma and biotech companies) and the large numbers of citations they receive, we feel that our work in these areas will be of continued relevance to a wide community of researchers.

Last updated on 2017-31-03 at 12:58