Regulation of nucleocytoplasmic transport in Drosophila


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2007
End date: 31/12/2009
Funding: 2700000 SEK


Description

The control of nucleocytoplasmic shuttling of gene regulators is a key aspect in the activation of signal transduction pathways. We have identified a complex of nuclear pore proteins, which tether the CRM1 export factor on the nuclear membrane and attenuate the rates of nuclear protein export. The analysis of mutants in genes encoding Nup88 and Nup214 shows that they are selective mediators of the nuclear translocation of NFkB proteins upon Toll signaling in Drosophila larvae. The work uncovers a new mechanism, whereby the attenuation of CRM1-export rates controls the nuclear translocation of signaling effectors and the activation of downstream genes. We have completed a comprehensive RNAi screen for nucleoporin function in protein nucleocytoplasmic transport. We discovered a new role of RanBP3 in the control of CRM1 localization and nuclear protein export. We also found two new components of the NPC with selective roles in the nuclear accumulation of the NFkB homolog , Dorsal in S2 cells. With a combination of genetic and biochemical approaches we aim to: A) Investigate the regulation and composition of the Nup88-Nup214 complex during signaling events. B) Explore the roles of Nup188, Nup155 and RanBP3 in NF?B protein translocation and Drosophila development.

Last updated on 2017-31-03 at 12:58

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