Chemical biology studies of protein-lipid interactions


Project leader


Funding source

Swedish Foundation for Strategic Research - Stiftelsen för strategisk forskning


Project Details

Start date: 01/08/2009
End date: 31/08/2012
Funding: 2100000 SEK


Description

The project aims to combine (i) an in vitro transcription/translation system, developed by the Swedish partner, that makes it possible to measure the free energy of interaction between amino acids incorporated into a transmembrane alpha-helix and the endoplasmic reticulum membrane, with (ii) the novel Flexizyme technique developed by the Japanese partner that allows an essentially unlimited variation of non-proteinogenic synthetic amino acids to be charged onto a suppressor tRNA. By using the charged suppressor tRNA to suppress a stop codon engineered into a defined position within the part of the gene that encodes the transmembrane helix, the non-proteinogenic amino acid can be incorporated at will and its effect on membrane integration of the helix can be measured in a true biological context. We will explore a wide range of side-chain structures in an attempt to develop a fragment-based free energy scale that describes the membrane affinity of different kinds of groups found in drug-like molecules and that can illuminate the interaction energetics between biomolecules and the endoplasmic reticulum membrane.

Last updated on 2017-29-03 at 17:13