Activation of B lymphocytes: adhesion, motility and regulation of Ig production


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2010
End date: 31/12/2012
Funding: 2100000 SEK


Description

B lymphocytes constitute an important part of the adaptive immune defense. The cells change adhesive and migratory properties, switch immunoglobulin (Ig) class and somatically mutate their V-genes during antigen activation in vivo. I wish to elucidate the mechanisms of B cell activation. Specific aims: -To study molecular mechanisms for B lymphocyte motility and adhesion in vitro and in vivo; -To study the role of the transcription factor Stat3 for regulation of IgE production, specifically in relation to the Hyper-IgE syndrome; -To study the role of the microflora for IgE production; -To set up a polyclonal in vitro system to study AID-induced somatic hypermutation of V-genes. The following methods will be used: Cell culture, molecular cloning, gene expression via retrovirus and lentivirus vectors, 2-photon laser scanning microscopy, making of monoclonal antibodies, making conditional knockout mice. We have previously studied B cell responses in the absence of the Wiskott Aldrich Syndrome protein in mice and in humans. We found that adhesion and motility was affected as well as the humoral immune response. We have made transgenic mice expressing different Ig genes and gained knowledge of how mutations are recruited to these regions. We have identified a number of genes, which are differentially expressed in activated B cells. This proposal has the focus on basic immunology, but the results can become important for the knowledge in immunodeficiencies, allergy and cancer.

Last updated on 2017-31-03 at 12:58