Intracellular protein transport in plants: signal peptides, molecular chaperones and proteolysis


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2016
End date: 31/12/2018
Funding: 2400000 SEK


Description

Organelle biogenesis requires specific intracellular protein transport, sorting, processing and assembly machineries. Newly synthesized organellar proteins contain targeting signals that are recognized by organellar receptors and mediate import into the organelle. Molecular chaperones assist protein import. We combine biochemical and molecular biological approaches with bioinformatics to study intracellular protein transport in plants with emphasis on sorting mechanisms and proteolysis. The project has resulted in several original findings revealing unique features of the plant mitochondrial protein import system. Our studies comprise 1) Molecular mechanisms of sorting of the nuclear encoded precursor proteins between mitochondria and chloroplasts, and dual targeting of proteins to both organelles mapping import determinants and interaction of signal peptides with organellar receptors. 2) Studies of novel cytosolic molecular chaperones and other protein recognising factors involved in protein sorting. 3) Organellar quality control mechanisms including studies of mammalian peptidasome hPreP and neurolysin, which are homologs of previously identified by us in Arabidopsis mitochondrial och chloroplastic peptidasome PreP and the novel oligopeptidase OOP, respectively. These studies will include effect of siRNA-mediated silencing of hPreP and neurolysin in human cell cultures and relation to dysfunction of mitochondria as well as investigation of amyloid-beta degradation by hPreP and neurolysin and possible role of these peptidases in Alzheimer ́s disease.


Last updated on 2017-28-07 at 09:56