Molecular studies of proteins in live cells


Project leader


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2016
End date: 31/12/2018
Funding: 2450000 SEK


Description

Although protein folding and function have been well explored under simplified conditions in vitro, it is yet unclear how these basic self-organisation events are modulated by the complex interior of live cells. The reason is that our current methods for analysing proteins at atomic-level resolution are not easily adaptable to in-vivo conditions with exceedingly low target-protein concentrations, molecular heterogeneity and overwhelming background ‘noise’. As outlined in this proposal, we have now cracked the basal part of these experimental problems by a combination of high-resolution resolution in-cell NMR, protein re-design, and quantitative analysis of tissue levels and aggregation kinetics of proteins in transgenic ALS mice. This allows us to address fundamental questions about the physical-chemical environment presented by the cellular interior and how this varies with physiological perturbations, as well as with evolutionary divergence.


Last updated on 2017-28-07 at 07:35