New systems for exposing human 3D-models of airway mucosa in health and disease to particulate- and gaseous air pollutants


Co-Investigators


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/01/2015
End date: 31/12/2017
Funding: 2040000 SEK


Description

The primary aim is to develop and evaluate unique and relevant normal and chronic bronchitis-like airway wall models with primary human bronchial cells from healthy subjects and smokers with and without COPD. The sophisticated airway wall models will be co-cultured with innate effector cells (neutrophils and alveolar macrophages) from different patient groups and mimic the in vivo-situation better than animal models where species differences always is an issue. These models with multiple cell types enable us to study cell-to cell interactions and cross-talk between the cells. By using our advanced exposure systems we can expose cell cultures to airborne particles, only consuming minimal amounts of test substance if the access is limited. Our chosen test substances of different ambient air pollutants like biodiesel particles, nanoparticles and aldehydes contribute to the development of asthma, chronic bronchitis, COPD and cardiovascular diseases and trigger symptoms in subjects suffering from those diseases. Development and validation of these models enable us to develop an in vitro testing strategy in order to reduce the requirement for animal inhalation studies. The applicant team has long experience of studies of in vivo-effects induced by exposure in an exposure chambers of the above mentioned agents in healthy subjects and subjects with respiratory diseases. Therefore the clinical relevance of the models will be well evaluated.


External Partners


Last updated on 2017-31-07 at 12:59