Cryo-EM analysis of the yeast heptameric post-translational translocon in a native membrane environment


Funding source

Swedish Research Council - Vetenskapsrådet (VR)


Project Details

Start date: 01/07/2016
End date: 30/06/2019
Funding: 3150000 SEK


Description

One of the initial steps in the biogenesis of secretory proteins is translocation through the Sec61 translocon at the endoplasmic reticulum (ER). In yeast, two alternative translocation pathways lead to the translocon: the well characterized co-translational pathway and the more obscure post-translational pathway. The post-translational pathway is an important route for many proteins and it has been shown that mutations in this machinery are directly related to complications and disease such as tumors in the gastric, colorectal and prostate systems. Therefore, the aim of this project is to address central questions regarding the post-translational translocon with the major focus on structural studies by single particle cryo-electron microscopy (cryo-EM). During the past two years, cryo-EM has faced great developments making it a promising alternative to X-ray crystallography, specifically for membrane proteins and protein complexes. My post-doctoral aim is to extend my experience in the field of structural biology by learning single particle cryo-EM and 3D reconstruction. To realize this aim, I will spend two years in the laboratory of Prof. Beckmann at the Ludwig Maximilian’s University of Munich. The laboratory is one the leading structural biology sites, offering great expertise and state-of-the-art instruments for structural and functional investigations of macromolecular machineries. This arrangement will offer me a powerful advantage on my return to Sweden.


Last updated on 2017-31-05 at 14:15